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ORIGINAL ARTICLE
Year : 2015  |  Volume : 13  |  Issue : 2  |  Page : 35-39

Prevalence and risk factors of bacterial vaginosis in a cohort of women seeking child spacing services in nigeria


Department of Obstetrics and Gynaecology, University of Port Harcourt Teaching Hospital, Port Harcourt, Rivers State, Nigeria

Date of Web Publication21-Nov-2015

Correspondence Address:
Nyengidiki Kennedy Tamunomie
Department of Obstetrics and Gynaecology, University of Port Harcourt Teaching Hospital, Port Harcourt, Rivers State
Nigeria
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1118-4647.170150

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  Abstract 

Background: The sustenance of reproductive potential of patients seeking contraceptive options is essential. The risk of bacterial vaginosis is increased by the choice of contraceptive options, which may affect postcontraception fertility. Objective: To determine the prevalence and risk factors of bacterial vaginosis in a cohort of women seeking child spacing services. Materials and Methods: A cross-sectional prospective study of women seeking fertility regulation services was conducted in a tertiary health facility in Nigeria between March and September 2014. Interviews were conducted to collect data on sociodemographic characteristics, characteristics of vaginal discharge, and sexual practices engaged by these women. Bacterial vaginosis was diagnosed using the Amsel criteria. Odds ratios (ORs) with 95% confidence intervals (95% CIs) were calculated to assess the predisposing factors. Results: One-hundred seventy-eight clients were recruited. Bacterial vaginosis was noted in 7.87% of these women. Patients had increased risk of bacterial vaginosis when they were of low socioeconomic class (OR 8.17; 95% CI 2.30–29.81), used vaginal drying agents (OR 9.70; 95% CI 2.35–46.15), had an early sexual debut (OR 9.56; 95% CI 2 54–38.92), a history of previous sexually transmitted infections (OR 21.39; 95% CI 4.99–105.30), and practiced vaginal douching (OR 19.23; 95% CI 3.82–130.43). Conclusion: The prevalence of bacterial vaginosis in this cohort raises the need for a high index of clinical suspicion in patients seeking fertility regulation services in the presence of notable risk factors. Avoidance of contraceptive methods that may increase the risk of bacterial vaginosis and the need for treatment to maintain reproductive potentials is advised.

Keywords: Bacterial vaginosis, fertility regulation, Nigeria


How to cite this article:
Tamunomie NK, Bassey G. Prevalence and risk factors of bacterial vaginosis in a cohort of women seeking child spacing services in nigeria. Niger J Gen Pract 2015;13:35-9

How to cite this URL:
Tamunomie NK, Bassey G. Prevalence and risk factors of bacterial vaginosis in a cohort of women seeking child spacing services in nigeria. Niger J Gen Pract [serial online] 2015 [cited 2019 Sep 23];13:35-9. Available from: http://www.njgp.org/text.asp?2015/13/2/35/170150


  Introduction Top


Bacterial vaginosis is the most common vaginal syndrome affecting premenopausal and pregnant women with an incidence of 20–50%.[1] About half of women with bacterial vaginosis may go through their reproductive career without manifestation of the symptoms of the disease.[2],[3] The female reproductive system has a natural mechanism of protecting itself by maintaining a specific ratio of lactobacilli flora, guarding against the pathogenic strains of bacteria, and ensuring a sustainable reproductive career.[4],[5] The number of lactobacilli spp. in the vagina is largely determined by hormonal, environmental factors, and sociocultural practices.[6] The alteration of the normal vaginal milieu results in the distortion of the delicate balance of the vagina by organisms such as gardnerella vaginalis, mobiluncus curtisii, clostridia, mycoplasma hominis, anaerobic Gram-negative rods, and peptostreptococcus species.[7],[8],[9] The presence of such organisms also causes alteration of the vagina pH >4.5, creating a creamish vaginal discharge with the characteristic fishy smell, and the presence of "clue cells" on microscopic examination of discharge. These stated conditions constitute the Amstel diagnostic criteria for bacterial vaginosis.[10]

Certain factors had been implicated in the etiology of bacterial vaginosis, and the influence of sociocultural practices such as vaginal douching, use of vaginal tightening agents among other risk factors had been highlighted in various reviews.[11],[12] Its association with tubal infertility, miscarriages, preterm rupture of fetal membrane, chorioamnionitis, and postpartum endometritis, and the development postoperative cuff infections during gynecological procedures is also well-documented.[13],[14],[15]

A woman's ability to reproduce, most instances, reduces the surveillance on factors affecting future fertility, and negative reproductive outcome. The family planning clinic provides fertility regulation services for the purpose of child spacing. Thus, having knowledge of the presence of bacterial vaginosis in patients who still want to reproduce and treating them will go a long way in avoiding the long-term sequelae of infertility, which is associated with bacterial vaginosis. Certain family planning methods have been associated with increased risk of bacterial vaginosis such as the intrauterine contraceptive device [16] while hormonal, and condom have a reverse predisposition to its occurrence.[17],[18]

It is on the basis of the above, we seek to know the prevalence of bacterial vaginosis which may be associated with adverse outcome in a perceived population of healthy women, who would still desire future child bearing.


  Materials and Methods Top


A cross-sessional prospective study was carried out at the University of Port Harcourt Teaching Hospital, Nigeria, between March 3 and September 30, 2014. All consecutive women who presented for fertility regulation for child spacing at the family planning clinic of the hospital were recruited. Women who presented for fertility limitation, who had had metronidazole or clindamycin for a disease condition other than bacterial vaginosis within the previous 90 days, irrespective of the route of administration or had experienced vaginal bleeding were excluded from the study.

Informed consent was obtained from all clients recruited, and ethics clearance for the study was obtained from the ethics review committee of the hospital.

Each client was interviewed using a prestructured questionnaire that was completed by the interviewers; information about age, tribe, occupation, level of education, husband's occupation, and parity were obtained. The social class of the patient was determined as proposed by Olusanya et al.[19] Other collected information included the history of abnormal vaginal discharge, practice of vaginal douching, use of vaginal tightening or drying products, and age of sexual debut. The number of lifetime sexual partners, history of sexually transmitted infections (STIs), and history of smoking were also ascertained.

Following the interview, each participant had a vaginal sample collected in the presence of a chaperon. Samples were collected with the aid of a wooden spatula from the vaginal walls and the posterior vaginal fornix.

The vaginal discharge or secretion was then assessed on the basis of the Amsel criteria. Visual inspection of the discharge looking for characteristics of bacterial vaginosis (homogenous and creamy) was done. The vaginal fluid pH was determined by adding 1 mL of normal saline to the collected vaginal secretion in a sterile test tube with a vaginal fluid pH of more than 4.5 suggestive of bacterial vaginosis using a pH stick. Thereafter, a drop of the solution was placed on a clean glass slide, and one drop of 10% potassium hydroxide in aqueous solution was added. This was done for all samples analyzed. The emission of a fishy odor ("whiff test") if present, is strongly suggestive of bacterial vaginosis. The presence of at least two out of three assessed criteria will warrant a microscopic examination of the prepared slide looking out for "clue cells." Confirmation of the diagnosis of bacterial vaginosis was made if more than 20% of the epithelial cells had adherent bacteria.

The sample size was determined using a formula proposed by Leshe-Kish for single proportions [20] and using a 9.8% bacterial vaginosis the prevalence of women attending family planning clinics.[21] The minimum sample size for this study was 136 clients but allowing an attrition of about 20%, a total of 178 clients were recruited.

The data were analyzed using SPSS version 15.0 (IBM, NY, USA) and Epi Info version 7.0 (Centers for Disease Control and Prevention, Atlanta, GA, USA). Frequency tables were generated with results tested for significance using the Student t-test and the χ2 test. P < 0.05 was considered statistically significant. An assessment of the risk factors for bacterial vaginosis among clients presenting for child spacing services was estimated by calculating odds ratios (ORs) with 95% confidence intervals (95% CIs).


  Results Top


A total of 178 women fertile women seeking fertility regulation services in the family planning clinic of the teaching hospital were recruited. Bacterial vaginosis was recorded among 14 (7.87%) of these women.

[Table 1] shows sociodemographic characteristics of the women. The mean age of the women was 26 ± 5 years with a range of 18–36. Twelve (85.7%) out of the 14 women with bacterial vaginosis were of low socioeconomic class. This was statistically significant. (P = 0.0000444, OR 8.17; 95% CI 2.30–29.81)
Table 1: Sociodemographic characteristics of clients

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[Table 2] shows the analysis of risk factors for bacterial vaginosis. In this population, a young age at sexual debut (15 and below) and a history of STIs were also both significantly associated with bacterial vaginosis (P = 0.0001612 and P = 0.0000010 each). Having had only one sexual partner was a protective factor, reducing the likelihood of bacterial vaginosis (P = 0.0000003). Smoking was not found to be associated with bacterial vaginosis in this population. Vaginal douching and the use of vaginal tightening and drying products were significantly associated with bacterial vaginosis among these women (P < 0.00000061 and P < 0.0002221 respectively).
Table 2: Risk factors for BV in women seeking child spacing services

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  Discussion Top


In this study, the prevalence of bacterial vaginosis among women seeking fertility regulation services was 7.8%. This value is relatively lower than the prevalence observed in the similar study population in Thailand where more than half of those diagnosed were asymptomatic.[22] The variation may be linked to the fact that study population was confined to those seeking family planning services for child spacing as against terminal contraception. This study, however, did not evaluate the symptomatology of the patients but is worthy of note that proper vigilance is necessary to identify patients with this condition so that the patient's condition are not aggravated by the choice of contraceptives. The significance of bacterial vaginosis in the population under discussion is buttressed by findings by Lassey et al. who noted bacterial vaginosis as the most common genital tract infection identified in women seeking contraceptive services in Ghana.[23] This study, however, did not investigate the various causes of discharge in the population studied. The disparity in the prevalence of bacterial vaginosis can be accounted for by the differences in the laboratory diagnostic criteria used for the two studies.

The association of low socioeconomic class to the development of bacterial vaginosis was highlighted in this study, where an eight-fold increase in the risk among the women studied was observed. This observation was corroborated by finding by other investigators.[24],[25] Persons of the lower socioeconomic class have been associated with poor health seeking, leaving untreated observed disease conditions because of economic limitations and high-risk sexual behavior which increases the risk of bacterial vaginosis which is a sexually related disease condition.[26]

The introduction of any substance into the vagina as evident by the practice of vaginal douching, and the use of drying agents are associated with a change in the vagina milieu.

Interior, increasing the alkalinity of the vagina and reducing the lactobacilli population. Among sexually active females in our environment, this practice is supposed to increase the sexual satisfaction of the male by encouraging vagina dryness with a false sense of tightness mimicking a virgin's vagina.[27] This study identified a strong association between these practices and bacterial vaginosis among the women, which is in keeping with previous studies.[28],[29] Keeping in view, the reason for the presentation of the patients at the family planning clinics, the choice of any contraceptive method that will worsen the incidence of bacterial vaginosis should be avoided in the selection of contraceptive options. The use of intrauterine contraceptive as an option in this group of patients was associated with an increased risk of bacterial vaginosis by Harikarnpukdee et al. and Ferraz et al.[30],[31]

During the period of adolescence, reproductive hormones, and genital tract anatomy increase the susceptibility of infections.[32] This survey identified an increased risk of bacterial vaginosis in women who had sexual exposure before the age of 15 years. At this age, the cylindrical epithelium is ectopic and are at greater risk of been affected by pathogenic organisms.[33] In addition to the above, patients in this age group are less likely to use a condom during sexual exposure which had been associated with reduced risk of contacting bacterial vaginosis.[32] Hence, condom use should be advised in women at this age to reduce the risk of bacteria vaginosis.

The relationship between sexual intercourse and bacterial vaginosis was further buttressed in this study, which noted that having one sexual partner is protective as against the having more than one. The involvement with more than one sexual partner indirectly is related to having increased frequency of intercourse and also the risk of sexually transmitted or enhanced infections which was also observed in the population studied with bacterial vaginosis. Patients with these attributes seeking contraceptive options should be screened for bacterial vaginosis while at the same should be offered contraceptive options not likely to increase bacterial vaginosis risk.

Varying opinions exist in the contribution of smoking to bacterial vaginosis with some in firm support of the positive link.[34],[35] Nicotine has been associated with reduced phagocytic properties and has anti-estrogenic properties, which reduces the lactobacilli population. Nevertheless, others reason that the smoking itself is a confounder to other high-risk sexual behaviors, which in turn increase the risk of bacterial vaginosis. These propositions were not supported by the findings of this study, which showed no association between bacterial vaginosis and smoking. This may be so because of the cultural background, which discourages smoking among the population. This may suggest that smoking is not a single significant factor that other cofactors must exist to facilitate the occurrence of bacterial vaginosis.

This study limited itself to identifying the prevalence of bacterial vaginosis among the subjects of interest; there may be need to further look into the prevalence of bacterial vaginosis among users of the various contraceptive options in a later survey.

Nevertheless, bearing in mind that women seeking child spacing services would need to maintain their reproductive potentials, it is imperative that reproductive limiting factors should be avoided.


  Conclusion Top


Identifying patients with the risk of bacterial vaginosis, treating them and avoiding contraceptive options that may likely aggravate the condition will go a long way in maintaining the postcontraceptive fertility of the patients. This will also assist in reducing the socioeconomic burden of preterm births and associated complications to the health system.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.

 
  References Top

1.
Allsworth JE, Peipert JF. Prevalence of bacterial vaginosis: 2001-2004 National Health and Nutrition Examination Survey data. Obstet Gynecol 2007;109:114-20.  Back to cited text no. 1
    
2.
Klebanoff MA, Schwebke JR, Zhang J, Nansel TR, Yu KF, Andrews WW. Vulvovaginal symptoms in women with bacterial vaginosis. Obstet Gynecol 2004;104:267-72.  Back to cited text no. 2
    
3.
McGregor JA, French JI. Bacterial vaginosis in pregnancy. Obstet Gynaecol Surv 2000;55:1-5.  Back to cited text no. 3
    
4.
Hillier SL. Diagnostic microbiology of bacterial vaginosis. Am J Obstet Gynecol 1993;169 (2 Pt 2):455-9.  Back to cited text no. 4
    
5.
Kumar N, Behera B, Sagiri SS, Pal K, Ray SS, Roy S. Bacterial vaginosis: Etiology and modalities of treatment – A brief note. J Pharm Bioallied Sci 2011;3:496-503.  Back to cited text no. 5
    
6.
Deborah BN, George M. Bacterial vaginosis in pregnancy: Current findings and future Directions. Epidemiol Rev 2002;24:102-8.  Back to cited text no. 6
    
7.
The Ten Teachers. Genital tract infection in gynaecology. In: Monga A, Dobbs S, editors. Gynaecology by Ten Teachers. 19th ed. Book Power; 2011. p. 52-3.  Back to cited text no. 7
    
8.
David N, Fredick L, Fiedler BS. Molecular identification of bacterial associated with bacterial vaginosis. N Engl J Med 2005;353:1899-911.  Back to cited text no. 8
    
9.
Hill GB. The microbiology of bacterial vaginosis. Am J Obstet Gynecol 1993;169 (2 Pt 2):450-4.  Back to cited text no. 9
    
10.
Sherrard J, Donders G, White D, Jensen JS; European IUSTI. European (IUSTI/WHO) guideline on the management of vaginal discharge, 2011. Int J STD AIDS 2011;22:421-9.  Back to cited text no. 10
    
11.
Mbizuo ME, Musya EE, Stray-Pedersen BF, Chinenje Z, Hussein A. Bacterial vaginosis and intravaginal practices: Association with HIV. Cent Afr J Med 2004;50:41-6.  Back to cited text no. 11
    
12.
Brotman RM, Klebanoff MA, Nansel TR, Andrews WW, Schwebke JR, Zhang J, et al. A longitudinal study of vaginal douching and bacterial vaginosis – A marginal structural modeling analysis. Am J Epidemiol 2008;168:188-96.  Back to cited text no. 12
    
13.
Eschenbach DA, Hillier S, Critchlow C, Stevens C, DeRouen T, Holmes KK. Diagnosis and clinical manifestations of bacterial vaginosis. Am J Obstet Gynecol 1988;158:819-28.  Back to cited text no. 13
    
14.
Mc Caffey M, Cottel E, Keane D. Bacterial vaginosis and infertility. Int J STD AIDS 1999;8:25-9.  Back to cited text no. 14
    
15.
Breton CF, Micheal GG. Other infectious conditions. In: James DK, Weiner CP, Steer PJ, Gonik B, editors. High Risk Pregnancy: Management Options. 3rd ed. Philadelphia: Elsevier; 2006. p. 689-90.  Back to cited text no. 15
    
16.
Madden L, Grentzer JM, Secura GM, Allsworth JE, Peipert JF. Risk of bacterial vaginosis in users of the intrauterine device: A longitudinal study. Sex Transm Dis 2012;39:217-22.  Back to cited text no. 16
    
17.
Van de Welgert JH, Vermijs MC, Turner AN, Morrison CS. Hormonal contraception may increase candidiasis: Implications for HIV transmission. AIDS 2013;27:2141-53.  Back to cited text no. 17
    
18.
Shoubnikova M, Hellberg D, Nilsson S, Ma'rdh PA. Contraceptive use in women with bacterial vaginosis. Contraception 1997;55:355-8.  Back to cited text no. 18
    
19.
Olusanya BO, Okpere E, Ezimokhai M. The importance of social class in voluntary fertility control in a developing country. West Afr J Med 1985;4:205-12.  Back to cited text no. 19
    
20.
Jekel JF, Katz DL, Elmore JG. Sample size, randomization and probability theory. In: Jekel JF, Katz DL, Elmore JG, editors. Epidemiology, Biostatistics and Preventive Medicine. 2nd ed. Philadelphia: WB Saunders; 2001. p. 196-204.  Back to cited text no. 20
    
21.
Cauci S, Driussi S, De Santo D, Penacchioni P, Iannicelli T, Lanzafame P, et al. Prevalence of bacterial vaginosis and vaginal flora changes in peri- and postmenopausal women. J Clin Microbiol 2002;40:2147-52.  Back to cited text no. 21
    
22.
Watcharotone W, Sirimai K, Kiriwat O, Nukoolkarn P, Watcharaprapapong O, Pibulmanee S, et al. Prevalence of bacterial vaginosis in Thai women attending the family planning clinic, Siriraj Hospital. J Med Assoc Thai 2004;87:1419-24.  Back to cited text no. 22
    
23.
Lassey AT, Newman MJ, Opintan JA. Vaginal flora of first time urban family planning attendants in Accra, Ghana. West Afr J Med 2005;24:219-22.  Back to cited text no. 23
    
24.
Bhalla P, Chawla R, Garg S, Singh MM, Raina U, Bhalla R, et al. Prevalence of bacterial vaginosis among women in Delhi, India. Indian J Med Res 2007;125:167-72.  Back to cited text no. 24
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25.
Begum A, Nilufar S, Akther K, Rahman A, Khatun F, Rahman M. Prevalence of selected reproductive tract infections among pregnant women attending an urban maternal and childcare unit in Dhaka, Bangladesh. J Health Popul Nutr 2003;21:112-6.  Back to cited text no. 25
    
26.
Ali CM, Sikdar TK, Sultana N, Ahmed N, Hague AK, Kameal SM, et al. Pattern of sexually transmitted diseases (STDs) among patients attending out patient department of dermatology of Dhaka Medical College Hospital, Dhaka, Bangladesh. J Dhaka Med Coll 2010;19:7-10.  Back to cited text no. 26
    
27.
Pitts M, Magunje N, McMaster J. Students' knowledge of the use of herbs and other agents as preparation for sexual intercourse. Health Care Women Int 1994;15:91-9.  Back to cited text no. 27
    
28.
Schwebke JR, Desmond RA, Oh MK. Predictors of bacterial vaginosis in adolescent women who douche. Sex Transm Dis 2004;31:433-6.  Back to cited text no. 28
    
29.
Turner AN, Morrison CS, Munjoma MW, Moyo P, Chipato T, van de Wijgert JH. Vaginal practices of HIV-negative Zimbabwean women. Infect Dis Obstet Gynecol 2010;2010. pii: 387671.  Back to cited text no. 29
    
30.
Ferraz do Lago R, Simões JA, Bahamondes L, Camargo RP, Perrotti M, Monteiro I. Follow-up of users of intrauterine device with and without bacterial vaginosis and other cervicovaginal infections. Contraception 2003;68:105-9.  Back to cited text no. 30
    
31.
Harikarnpukdee D, Sirimai K, Kiriwat O, Boriboonhirunsarn D. Prevalence of bacterial vaginosis among intrauterine device users in Thai women attending family planning clinic, Siriraj Hospital. J Med Assoc Thai 2004;87 Suppl 3:S8-11.  Back to cited text no. 31
    
32.
Mascarenhas RE, Machado MS, Costa e Silva BF, Pimentel RF, Ferreira TT, Leoni FM, et al. Prevalence and risk factors for bacterial vaginosis and other vulvovaginitis in a population of sexually active adolescents from Salvador, Bahia, Brazil. Infect Dis Obstet Gynecol 2012;2012:378640.  Back to cited text no. 32
    
33.
Krashin JW, Koumans EH, Bradshaw-Sydnor AC, Braxton JR, Evan Secor W, Sawyer MK, et al. Trichomonas vaginalis prevalence, incidence, risk factors and antibiotic-resistance in an adolescent population. Sex Transm Dis 2010;37:440-4.  Back to cited text no. 33
    
34.
Hay PE, Lamont RF, Taylor-Robinson D, Morgan DJ, Ison C, Pearson J. Abnormal bacterial colonisation of the genital tract and subsequent preterm delivery and late miscarriage. BMJ 1994;308:295-8.  Back to cited text no. 34
    
35.
Jonsson M, Karlsson R, Rylander E, Gustavsson A, Wadell G. The associations between risk behaviour and reported history of sexually transmitted diseases, among young women: A population-based study. Int J STD AIDS 1997;8:501-5.  Back to cited text no. 35
    



 
 
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